1705-P: Expression of Selected MicroRNAs in Adipose Tissue-Derived Mesenchymal Stem Cells and Metabolic Syndrome

Abstract

Adipose tissue mesenchymal stem cells (AdMSC) secrete microRNAs (miR). Although miR are involved in pathogenesis of metabolic syndrome, the role of those secreted by AdMSC remains elusive. We aimed to assess expression of selected miR by AdMSC in relation to basic metabolism parameters in nondiabetic adults. For this preliminary study 10 obese, 8 overweight and 5 normal weight subjects with no inflammatory or cancerous disease were enrolled, including 19 female and 6 prediabetic ones. AdMSC were harvested from abdominal subcutaneous fat and cultured in standard conditions. Expressions of four miR reported in metabolic disturbances were estimated with RT-qPCR. Total and visceral fat were assessed with bioimpedance. Expression of miR-21, miR-122, or miR-192 did not correlate with any clinical parameter. On the other hand, expression of miR-155 [2-ΔCt] was lowest in obese subjects (3.69±2.67 vs. 7.07±4.42 in overweight and 10.25±7.05 ×10-3 in normal weight ones, P =0.04, K-W ANOVA). It correlated inversely with BMI, total fat mass, visceral adiposity, and serum CRP, whereas positively (marginally significantly) with serum HDL-cholesterol (table 1). Expression of miR-155 in AdMSC appears blunted in visceral obesity, which correlates with HDL-dyslipidemia and microinflammation. This calls for further study of AdMSC miR secretome in the pathogenesis of metabolic syndrome with focus on miR-155. Disclosure G. Wystrychowski: Other Relationship; Polfa Tarchomin. K. Simka-Lampa: None. A. Witkowska: None. E. Sobecko: None. A. Skubis-Sikora: None. B. Sikora: None. E. Wojtyna: None. K. Gwizdek: None. A. Golda: Other Relationship; Servier Laboratories. M. Wróbel: Other Relationship; Novo Nordisk, Lilly, Boehringer Ingelheim Inc. C. Kruszniewska-Rajs: None. J. Gola: None. Funding Medical University of Silesia, Katowice, Poland