Abstract
While TransIT-LT1 is not quite as effective, TransfeX facilitates unusually high levels of transgene expression at levels comparable to that achieved by adenoviral transduction (our unpublished data). Transfection of HeLa cells with TransfeX exhibited the highest transfection activity obtained in our laboratory, and was almost 5 times higher than that achieved with TransIT-LT1. In HSC-3 cells, transfection efficiency of TransfeX was 29 times higher than that of TransIT-LT1. Very high levels of luciferase activity were obtained in H357 cells with TransfeX. TransfeX is likely to be beneficial in various cancer gene therapy approaches and other gene therapies involving non-viral vectors.
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