170 IMPACT OF PERIPROSTATIC IMPLANTATION OF BONE MARROW-DERIVED, MESENCHYMAL STEM CELLS ON THE RECOVERY OF PENILE ERECTION IN A RAT MODEL OF CAVERNOUS NERVE INJURY

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You have accessJournal of UrologyStem Cell Research1 Apr 2011170 IMPACT OF PERIPROSTATIC IMPLANTATION OF BONE MARROW-DERIVED, MESENCHYMAL STEM CELLS ON THE RECOVERY OF PENILE ERECTION IN A RAT MODEL OF CAVERNOUS NERVE INJURY Dalsan You, Kwang Hyun Kim, Myoung Jin Jang, Jiyeon Lee, Kyung Hyun Moon, Young Hwan Ji, In Gab Jeong, Tai Young Ahn, and Choung-Soo Kim Dalsan YouDalsan You Seoul, Korea, Republic of More articles by this author , Kwang Hyun KimKwang Hyun Kim Seoul, Korea, Republic of More articles by this author , Myoung Jin JangMyoung Jin Jang Seoul, Korea, Republic of More articles by this author , Jiyeon LeeJiyeon Lee Seoul, Korea, Republic of More articles by this author , Kyung Hyun MoonKyung Hyun Moon Ulsan, Korea, Republic of More articles by this author , Young Hwan JiYoung Hwan Ji Ulsan, Korea, Republic of More articles by this author , In Gab JeongIn Gab Jeong Seoul, Korea, Republic of More articles by this author , Tai Young AhnTai Young Ahn Seoul, Korea, Republic of More articles by this author , and Choung-Soo KimChoung-Soo Kim Seoul, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.239AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Post-prostatectomy erectile dysfunction is a common complication of radical prostatectomy (RP) and it decreases the quality of life of the patient. We evaluated the effect of periprostatic implantation of mesenchymal stem cells (MSC) on the recovery of penile erection in a rat model of CN injury. METHODS Forty, eight-week-old, male Sprague-Dawley rats were randomly divided into four groups (ten rats per group) as follows: 1) only laparotomy (sham group); 2) bilateral CN dissection (BCND) and 0.1 mol/L phosphate-buffered saline instillation (injured control group); 3) BCND and fibrin glue instillation (fibrin glue group); and 4) BCND and periprostatic implantation of MSC with fibrin glue (MSC group). Four week following implantation, systemic arterial pressure (AP) and intracavernosal pressure (ICP) were recorded. To compare erectile function among the groups, the maximal ICP (ICPmax) was divided by the simultaneous AP (ICPmax/AP). After the function evaluation, the penis and bilateral prostate lobes of each rat were harvested and cryopreserved in liquid nitrogen for cGMP expression analysis and observation of nanoparticle-tagged MSC, respectively. RESULTS Mean ICPmax/AP ± S.E.M was significantly decreased in the injured control and fibrin glue groups (23.2% ± 7.1 and 24.4% ± 5.3, respectively) compared with that of the sham group (64.2% ± 5.0)(p=0.001 and p<0.001, respectively). Mean ICPmax/AP of the MSC group (41.5% ± 4.2) was lower than that of the sham group (p=0.005), although it was higher than those of the injured control and fibrin glue groups (p=0.010 and p=0.023, respectively). Mean cGMP ± S.E.M was decreased in the injured control and fibrin glue groups (5.27 fmol/mg ± 0.26 and 5.03 fmol/mg ± 0.22, respectively) compared with that of the sham group (5.87 fmol/mg ± 0.19)(p=0.126 and p=0.029, respectively). Mean cGMP of the MSC group (5.86 fmol/mg ± 0.25) was similar to that of the sham group (p=0.905). From the harvested prostate, merging of DAPI (4',6-diamidino-2-phenylindole) and nanoparticle was identified, indicating the presence of viable MSC. In addition, several MSCs were stained with p75 antibody, a marker of Schwann cells. CONCLUSIONS Following the periprostatic implantation of MSC in a rat model with CN injury, the recovery of penile erection was noted. The possible mechanism might include the differentiation of MSC into Schwann cells and resulting in inhibiting demyelination of the CN and assisting in axonal regeneration. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e71 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dalsan You Seoul, Korea, Republic of More articles by this author Kwang Hyun Kim Seoul, Korea, Republic of More articles by this author Myoung Jin Jang Seoul, Korea, Republic of More articles by this author Jiyeon Lee Seoul, Korea, Republic of More articles by this author Kyung Hyun Moon Ulsan, Korea, Republic of More articles by this author Young Hwan Ji Ulsan, Korea, Republic of More articles by this author In Gab Jeong Seoul, Korea, Republic of More articles by this author Tai Young Ahn Seoul, Korea, Republic of More articles by this author Choung-Soo Kim Seoul, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...