Abstract
A PCR-based telomeric repeat amplification protocol (TRAP) determines the telomerase activity in many human immortal cell lines, normal germline and somatic tissues, and biopsy samples from various types of tumors. Studying these telomerase activities may be helpful in various clinical issues. And quantification of the level of telomerase activity becomes necessary to study the clinical association between telomerase activity and tumor progression. Several quantitative TRAP methods have been used for small clinical studies. This chapter describes the conversion of gel-based TRAP to the kinetic format of TRAP. This simple, fast, nonradioactive, and quantitative format requires very little hands-on time and is suitable for analyzing a large number of samples. The chapter discusses associated protocols in details. And finally, the results are demonstrated with figures to conclude the discussion. The kinetic TRAP is simple, sensitive, nonradioactive, accurate, and requires little hands-on time after amplification. Also, this quantitative format is appropriate for screening a large number of clinical specimens to demonstrate the clinical utility of using telomerase as a diagnostic and prognostic tumor marker.
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