17α-Methyltestosterone implants accelerate spermatogenesis in common snook, Centropomus undecimalis, during first sexual maturation

Abstract

The common snook, Centropomus undecimalis, is an emerging species for intensive fish culture, however, some reproductive aspects of this species, especially the development of the testes and the action of androgen hormones on spermatogenesis have not been studied. The objective of this study was to evaluate the effects of 17α-methyltestosterone (MT) on spermatogenesis and steroidogenesis during the first sexual maturation of the common snook. The fish, which were reproduced in captivity, had a body weight of 305.80 ± 35.60 g and a total length of 34,11 ± 1,08 cm. We used ethylene-vinyl-acetate (EVAc) implants with four concentrations of the hormone MT: T1 (0.3 mg/kg); T2 (3.0 mg/kg); T3 (15.0 mg/kg) and T4 (30.0 mg/kg), and a control group that did not receive the hormone. The gonads increased (P < 0.05) in relation to the concentrations of MT. Histological analysis revealed a progression of spermatogenesis in the MT treatments, especially in T3 and T4. Sperm release was attained in some fish treated with MT. However, there was a partial suppression of the levels of testosterone (T) and 11-ketotestosterone (11-KT) in plasma in the MT treatments, indicating a negative feedback on steroidogenesis. However, this suppression of T and 11-KT in plasma did not prevent an increase in the gonadosomatic index and the progression of gametogenesis. There was also an increase of estradiol (E2) in plasma in the treatments with the highest MT concentrations. The results suggest that the application of EVAc implants with MT at concentrations of 15 and 30 mg/kg stimulates the development and growth of the testes and accelerates spermatogenesis.