1.7 - A Biosensor for Simultaneously Determining The L-Glutamate and Glucose in The Fermentation Process

Abstract

This chapter elaborates a biosensor for simultaneously determining the l-glutamate and glucose in the fermentation process. The control of l-glutamate fermentation process needs rapid detection of both production of l-glutamate, and consumption of glucose. l-Glutamate oxidase, and glucose oxidase were separately immobilized on polycarbonate membranes with bovine serum albumin by using bifunctional agent, glutaraldehyde. The immobilized enzyme membranes were separately placed on the sensing area of two H2O2 electrodes, and set in the same reaction chamber. The whole configuration consisting of two oxidase membrane-H2O2 electrodes, enzyme reaction system and computer control system, has been marketed as the model SBA-40 functional analyzer. The important feature of this system is to allow the two different substrates to be detected simultaneously and rapidly without interference of each other. The analyzer exhibits many advantages such as signal treatment, data display, function control, and autoprint. The optimal selection of phosphate system for the oxidase membranes was at pH 7.0. Each linear range was between 0 and 100 mg/dl with a response of 20 s, and 25 μ samples each assay.