Abstract
The aim of the study was to assess the usefulness of 16S rRNA gene in identification of Brucella spp. and phylogenetic study. For identification purposes, a 1477-bp fragment of 16S rRNA gene of 16 isolates and 3 control reference strains was amplified and 3 isolates with B. abortus S19 sequenced. The obtained sequences were submitted to GenBank for species identification. Sequence analysis of a 1477-bp 16S rRNA fragment allows the identification of Brucella spp. However, for discrimination of closely related species sequencing of the entire 16S rRNA gene, additional sequencing of the 23S rRNA gene or sequencing of the 16S-23S rRNA intergenic spacer is essential.
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