Abstract
We report here 16S rRNA-based bacterial diversity existing during freezing conditions in a high-altitude Himalayan lake through sequencing a 16S rRNA gene amplicon data set. A total of 121,857 high-quality reads were obtained; 40.78% of the bacterial population was classified to the genus level, while 1.26% was classified to the species level.
Highlights
We report here 16S rRNA-based bacterial diversity existing during freezing conditions in a high-altitude Himalayan lake through sequencing a 16S rRNA gene amplicon data set
The HQ reads were subjected to operational taxonomic unit (OUT) identification at 97% sequence similarity and taxonomic assignment of OTUs using the Greengenes database (16S/Archaea database) and the Quantitative Insights into Microbial Ecology (QIIME) module
Krona-based [9] diagram visualization showed that 99.22% of the microbially diverse population represents Bacteroidetes (35.97%), Firmicutes (31.27%), Proteobacteria (22.56%), Tenericutes (8.38%), and Planctomycetes (1.04%) at the phylum level (Fig. 1)
Summary
We report here 16S rRNA-based bacterial diversity existing during freezing conditions in a high-altitude Himalayan lake through sequencing a 16S rRNA gene amplicon data set. (i.e., those with a more than 10% quality threshold [QV] of Ͻ20 Phred quality score). A total of 121,857 high-quality reads were obtained.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
