16S rDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome.

Sitong Dong,Da Li,Gaoyu Li,Zhen Wang,Kai Yang,Chao Liu,Jiao Jiao,Wei Zhang,Shuangshuo Jia,Xiuxia Wang

doi:10.3389/fcimb.2021.634981

Sitong Dong, Da Li + Show 8 more

Open Access

https://doi.org/10.3389/fcimb.2021.634981

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Abstract

ObjectiveTo study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).MethodWe recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).ResultWe found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with Prevotella enterotype when compared to control women with Prevotella enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with Bacteroides enterotype. Ruminococcus gnavus, Prevotella stercorea, Dialister succinatiphilus and Bacteroides fragilis were more abundant while Christensenellaceae spp. were less abundant in the PCOS group. P. stercorea was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.ConclusionOur study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS via drugs and diet.

Highlights

Polycystic ovary syndrome (PCOS) is a reproductive endocrine disorder affecting 5%–10% of women of reproductive age (Aversa et al, 2020)
PRL, sex hormone-binding globulin (SHBG), and Follicle-stimulating hormone (FSH) were lower, while Free Androgen Index (FAI), TT, luteinizing hormone (LH), free triiodothyronine 3 (FT3), fasting plasma insulin (FINS), Anti-Müllerian hormone (AMH), and insulin resistance (IR) were higher in the PCOS group (Supplementary Table 2)
We noticed a trend that patients in PCOS-IR and PCOS-OW groups showed more disturbances in biochemical indexes, FINS, fasting plasma glucose (FPG), Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), and TT when compared to PCOS-NIR and PCOS- groups (Supplementary Table 2), though no significant statistical difference was observed

Summary

Introduction

Polycystic ovary syndrome (PCOS) is a reproductive endocrine disorder affecting 5%–10% of women of reproductive age (Aversa et al, 2020). The pathophysiological mechanism of PCOS is currently unclear. It is often characterized by polycystic ovarian changes, oligomenorrhea, and elevated androgen levels. Some patients can have metabolic syndrome with various clinical manifestations accompanied by obesity, type 2 diabetes, hyperlipidemia, insulin resistance hypertension, and high-risk cardiac factors for cerebrovascular disease. Emerging evidence shows many metabolic diseases, such as type 2 diabetes, nonalcoholic fatty liver, insulin resistance, and cardiovascular diseases (Qin et al, 2012; Qin et al, 2014; Jie et al, 2017; Woodhouse et al, 2018; Pierantonelli and Svegliati-Baroni, 2019), are related to changes in the gut microbiota spectrum

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