1689 THE EFFECT OF VACUUM THERAPY ON PEYRONIES' DISEASE RAT MODEL

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Peyronie's Disease/Surgical Therapy I1 Apr 20121689 THE EFFECT OF VACUUM THERAPY ON PEYRONIES' DISEASE RAT MODEL Haocheng Lin and Run Wang Haocheng LinHaocheng Lin Houston, TX More articles by this author and Run WangRun Wang Houston, TX More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1584AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Vacuum therapy has recently been used by some investigators as a therapy for peyronie's disease (PD). However there is no scientific evidence supporting its use at present time. This study was designed to use our rat-specific vacuum erection device (VED) in rat PD model to evaluate the effect of vacuum device therapy on PD. METHODS PD animal model was established following intra-tunical injection of Transforming growth factor beta-1 (TGFβ1) and Thrombojet carrier solution on one side of cavernosal body. Adult Sprgue-Dawley rats were randomly assigned into three groups: Group1: Control-saline was injected into tunica albuginea (TA). Group 2: PD model-TGFβ1 and Thrombojet were injected into TA. Group 3: PD model with VED treatment-TGFβ1 and Thrombojet were injected into TA followed by VED therapy after the PD model was successfully established. VED was applied under isoflurane inhalation, 5 min twice daily with a 1 min interval, Monday–Friday. Penile curvature angle was measured weekly. After 4 weeks treatment, intracavernosal pressure (ICP) and mean arterial pressure (MAP) are measured under cavernous nerve stimulation, the penis was collected and processed for immunohistochemistry of TGFβ1, Inducible nitric oxide synthase (iNOS) and Terminal deoxynucleotidyl transferase dUTP nick end labeling assay (TUNEL). RESULTS 1. Histology stain and penile deformity confirmed PD rat model was successfully established. 2.VED therapy reduced the penile curvature angle in PD rat model. 3. VED therapy improved erectile function in PD rat model as demonstrated by ICP/MAP ratio. 4. VED therapy decreased TGFβ1 and iNOS expression level. 5. VED therapy decreased cell apoptotic in corpus cavernosum of PD rats. CONCLUSIONS Our study demonstrated that VED therapy was effective to reverse PD progression and preserve erectile function. The underlying mechanisms might be mechanic distraction, anti-apoptosis and anti-fibrosis. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e682 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Haocheng Lin Houston, TX More articles by this author Run Wang Houston, TX More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...