1683 EFFECT OF DETRUSOR OVERACTIVITY INDUCED BY BLADDER OUTLET OBSTRUCTION ON INTERSTITIAL CELLS IN THE URINARY BLADDER OF RATS

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Non-Neurogenic Voiding Dysfunction II1 Apr 20101683 EFFECT OF DETRUSOR OVERACTIVITY INDUCED BY BLADDER OUTLET OBSTRUCTION ON INTERSTITIAL CELLS IN THE URINARY BLADDER OF RATS Byung Seok Oh, Kim Sun-Ouck, Jun Back Park, Chang Min Im, Kyung Jin Oh, Seung Il Jung, Taek Won Kang, Kwon Dongdeuk, Park Kwangsung, and Soo Bang Ryu Byung Seok OhByung Seok Oh More articles by this author , Kim Sun-OuckKim Sun-Ouck More articles by this author , Jun Back ParkJun Back Park More articles by this author , Chang Min ImChang Min Im More articles by this author , Kyung Jin OhKyung Jin Oh More articles by this author , Seung Il JungSeung Il Jung More articles by this author , Taek Won KangTaek Won Kang More articles by this author , Kwon DongdeukKwon Dongdeuk More articles by this author , Park KwangsungPark Kwangsung More articles by this author , and Soo Bang RyuSoo Bang Ryu More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1508AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Recent studies have shown that interstitial cells (ICs) are widely distributed in the genitourinary tract, and their involvement in spontaneous electrical activity and muscle contraction has been suggested. However, nitric oxide (NO) is thought to play a role in bladder overactivity related to partial bladder outlet obstruction (BOO). The purpose of this study was to investigate the effect of BOO on ICs and nitric oxide synthase (NOS) isoforms in the urinary bladder of rats. METHODS Female Sprague-Dawley rats (230-240 g, n=60) were divided into 2 groups: a control group (group Con, n=30) and a group that underwent partial BOO (group BOO, n=30). After 4 weeks, a urodynamic study was conducted to measure the contraction interval and contraction pressure. The expression and cellular localization of ICs, endothelial NOS, and neuronal NOS in the urinary bladder of rats were determined by Western blot and immunohistochemical analysis, respectively. RESULTS Four weeks after surgery, voiding frequency increased and voiding volume decreased in group BOO. Filling cystometry showed a reduction in the interval between voiding contractions and an increase in voiding pressure in the bladders of group BOO. The estimated contraction interval (min, mean ?SE) was significantly lower in group BOO (2.9?.35) than in group Con (6.1?.05) (p<0.05). The thickness of and the population of kit-positive ICs were significantly greater in the suburothelial and detrusor smooth muscle layers of bladders in group BOO than in group Con. ICs had close contact with each other and with neighboring neuronal NOS–expressing nerves. CONCLUSIONS The results indicate that the population of ICs is greater in rats with than in those without partial BOO and suggest that functional changes in ICs and NOS isoforms may contribute to the pathophysiology of bladder overactivity induced by BOO. Gwangju, Republic of Korea© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e650 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Byung Seok Oh More articles by this author Kim Sun-Ouck More articles by this author Jun Back Park More articles by this author Chang Min Im More articles by this author Kyung Jin Oh More articles by this author Seung Il Jung More articles by this author Taek Won Kang More articles by this author Kwon Dongdeuk More articles by this author Park Kwangsung More articles by this author Soo Bang Ryu More articles by this author Expand All Advertisement Advertisement Loading ...