Abstract
Objective: To investigate the effects of hibernation drugs on early wound sepsis in severely scalded rats. Methods: Sprague-Dawley rats inflicted 30% TBSA deep thickness were randomly divided into A (immediate resuscitation) and B (immediate resuscitation and lytic cocktail). After infusion of 108 CFU/ml Pseudomonas aeruginosa in the wound on third postburn day, survival was compared between two groups. Local tissue samples and plasma were collected and the plasma level of IL-2 mRNA, IL-4 mRNA, IL-10 mRNA, and IFN-γ mRNA were assayed by fluorescence PCR. Results: Compared with Group A, host survival significantly increased (0.663 ± 0.026, P < 0.01), viscera injury decrease in group B after infusion bacteria. The plasma Th1 cytokine mRNA (IL-2, IFN-γ) levels in B group were significantly increased compared with A group. However, the plasma Th2 cytokine mRNA (IL-4, IL-10) levels in group B were not as significantly increased as group A. Conclusions: Immediate resuscitation with hibernation drugs can decrease viscera injury after sepsis, increase host survival and improve host immune system by preventing Th1 cell from over activation and decrease Th2 cytokines.
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