1611 QUANTITATIVE MEASUREMENT OF THE ANDROGEN RECEPTOR IN PREPUCES OF BOYS WITH AND WITHOUT HYPOSPADIAS

Abstract

You have accessJournal of UrologyPediatrics: Congenital Anomalies - Lower Urinary Tract & Genitalia II1 Apr 20121611 QUANTITATIVE MEASUREMENT OF THE ANDROGEN RECEPTOR IN PREPUCES OF BOYS WITH AND WITHOUT HYPOSPADIAS Renate Pichler, Gabriel Djedovic, Helmut Klocker, Verena Hauser, Georg Schäfer, Christian Radmayr, Wolfgang Horninger, and Josef Oswald Renate PichlerRenate Pichler Innsbruck, Austria More articles by this author , Gabriel DjedovicGabriel Djedovic Innsbruck, Austria More articles by this author , Helmut KlockerHelmut Klocker Innsbruck, Austria More articles by this author , Verena HauserVerena Hauser Innsbruck, Austria More articles by this author , Georg SchäferGeorg Schäfer Innsbruck, Austria More articles by this author , Christian RadmayrChristian Radmayr Innsbruck, Austria More articles by this author , Wolfgang HorningerWolfgang Horninger Innsbruck, Austria More articles by this author , and Josef OswaldJosef Oswald Innsbruck, Austria More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1406AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The etiology of hypospadias is not yet understood. The aim of this study was to quantify and compare the androgen receptor (AR) mRNA and protein in prepuces of boys with and without hypospadias. METHODS 40 prepuce specimens of circumcised boys (20 with and 20 without hypospadias) were enrolled in this prospective study. Mean age was 61.8 (range 18 to 162) and 30.9 (range 13 to 156) months in children without and with hypospadias at time of surgery. Coronal, penile and sine hypospadias were seen in 9 (45%), 7 (35%) and 4 (20%) children respectively. Immediately after surgery tissue samples were divided, one part was fixed in formaldehyde and embedded in paraffin, the other one was snap frozen in liquid nitrogen at -80° C. Total RNA was isolated from frozen tissue and transcribed to cDNA. The amount of androgen receptor mRNA was measured by quantitative real time PCR. Western Blot and standardized, automated immunohistochemistry (Discovery XT, Ventana) were used to assess androgen receptor protein levels. RESULTS The androgen receptor mRNA expression was significantly elevated in the prepuces of boys with hypospadias compared to boys with phimosis (28.33±5.39 vs. 15.31±1.85, p<0.05). Furthermore, the amount of androgen receptor protein was higher in boys with, compared to boys without hypospadias (133.25±6.17 vs. 100±4.45, p<0.05). These results suggest that a decreased androgen receptor DNA binding and functional capability elicits a compensatory up-regulation of androgen receptor mRNA and protein. CONCLUSIONS Our results suggest that the different androgen receptor mRNA and protein expression levels indicate the extent of androgen receptor signaling defects in boys with hypospadias. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e652 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Renate Pichler Innsbruck, Austria More articles by this author Gabriel Djedovic Innsbruck, Austria More articles by this author Helmut Klocker Innsbruck, Austria More articles by this author Verena Hauser Innsbruck, Austria More articles by this author Georg Schäfer Innsbruck, Austria More articles by this author Christian Radmayr Innsbruck, Austria More articles by this author Wolfgang Horninger Innsbruck, Austria More articles by this author Josef Oswald Innsbruck, Austria More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...