160 Defocused Low-energy Shock Wave can Stimulate Penile Tissues to Produce more Energy for Growth and Proliferation in a DMED Rat Model

Abstract

Defocused low-energy shock wave therapy (DL-ESWT) is a novel and potential way in regeneration medicine, and now it has been used in many areas which have demonstrated its unique superiority. At present, some researchers have applied ESWT to clinical therapy for ED, which achieves satisfactory therapeutic effects. However, the metabolic change of corpus cavernosum with ESWT is still unclear. To investigate the metabolic change of corpus cavernosum with ESWT in a rat model of DMED induced by streptozotocin (STZ). We divided Human umbilical vein endothelial cells (HUVECs) into 3 groups. Group 1 is control group, without ESWT. Group 2 is half treated group, with ESWT for former 3 passages. Group 3 is full treated group, and we applied ESWT to HUVECs for every passage. After 5 passages, we collected the cells, supernatants and proteins for ELISA and Western blot. The DMED rats were randomly divided into 2 groups (N = 10 per group): 1 DMED group, 2 DMED+ESWT group. The normal group (N=10, without STZ) is the control group. Erectile function and other expression experiments were carried out after STZ injection of 8 weeks. ESWT was repeated 3 times per week with one day's break, for a total duration of 4 weeks. Immediately after recording of intracavernous pressure (ICP), the penis was then harvested for histologic analysis, ELISA and western blotting.