Abstract
Some Mold can proliferate in food to produce mycotoxin such as alphatoxin producing strain, Aspergillus flavus. Previous studies showed that the alphatoxin can cause cell mutation to induce hepatic cancers. Chemical preservative can be used in foodstuffs to inhibit mold proliferation. However, this method can let the chemical preservative remained to induce allergy and even harmful to health. Up to now, many studies found natural-non-toxic antimicrobial metabolites (biopreservatives) can be produced from some potential bacteria. Therefore, using biopreservatives to replace chemical preservatives against alphatoxin producing mold is important issue today. The Bacillus-like bacteria were isolate from health chicken intestines. After fermentation and acid precipitation, the crude CFS(cell free supernatant) extracts from various bacteria were obtained. These extracts from various bacteria can been determined whether can inhibit alphatoxin-produced fungi (Aspergillus flavus) by using agar well diffusion method. Thus the potential bacteria having anti-fungi activities were selected by agar well diffusion method. The potential bacteria strain can be further identified by 16S rRNA sequence, RAPD(random amplified polymorphic DNA) and API CH 50B method. The data from 16S rRNA sequence analysis reveal that the potential bacteria strain was Bacillus family. However the 16S rDNA analysis can't discriminate between Bacillus amyloliquefaciens and Bacillus subtilis. Thus the potential strain was further analyzed by using API CH 50 B biochemical identification system. The above result indicated the potential strain has 95% similarity to the Bacillus amyloliquefaciens. Next, analysis of RAPD pattern among the potential strain and reference strains of Bacillus amyloliquefaciens and Bacillus subtilis, the result demonstrated the potential bacteria strain, isolated from health chicken intestines with inhibition of alpha toxin producing fungi potential is Bacillus amyloliquefaciens.
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