Abstract
Choline kinase or ATP:choline phosphotransferase catalyzes the phosphorylation of choline with ATP yielding phosphorylcholine and ADP. This is the initial enzyme of the CDPcholine pathway, which serves as the principal pathway of phosphatidylcholine synthesis in animal tissues. Choline kinase has been highly purified from lung, kidney, liver and brain. The brain is one of the richest sources of choline kinase. The enzyme of the soluble fraction from brain has been purified to homogeneity and shown to mediate the phosphorylation of choline, N,N -dimethylethanolamine, N -monomethylethanolamine, and ethanolamine. Choline kinase activity is assayed by determining the rate of formation of either phosphorylcholine isotopically or ADP spectrophotometrically. In the first method, [ 14 C]choline is used as substrate and the phosphorylcholine formed is separated from unreacted choline by solvent extraction and then counted. In the second method, choline kinase is coupled with the pyruvate kinase–lactate dehydrogenase system, so that the ADP formed can be determined spectrophotometrically by measuring the decrease in NADH.
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