Abstract

SUMMARYThe lichens Lobaria pulmonaria and Lobaria oregana were assayed for 15N2 assimilation in order to confirm previous rates of nitrogenase activity measured by acetylene reduction. In addition, the effect of molybdenum on acetylene reduction activity was studied in both species. The molar conversion ratio (acetylene reduction rate divided by 15N2 fixation rate) was 4.27 for Lobaria pulmonaria and 4.78 for Lobaria oregana. The rate of 15N2 uptake decreased over time in both species. Addition of 1 p.p.m. molybdenum enhanced acetylene reduction by 180% in Lobaria pulmonaria and by 50% in Lobaria oregana. Optimum enhancement in Lobaria oregana was obtained with 1 p.p.m. molybdenum, while 10 p.p.m. may be inhibitory.

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