1590 DISCRIMINATION OF OAB FROM IC/PBS BY MULTIVARIATE DATA MODELING OF URINARY PROTEINS

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Incontinence - Evaluation & Therapy II1 Apr 20101590 DISCRIMINATION OF OAB FROM IC/PBS BY MULTIVARIATE DATA MODELING OF URINARY PROTEINS Pradeep Tyagi, Vikas Tyagi, Don Bui, Harvey Qu, Kenneth Peters, Yao-Chi Chuang, Hann-Chorng Kuo, Naoki Yoshimura, and Michael Chancellor Pradeep TyagiPradeep Tyagi Royal Oak, MI More articles by this author , Vikas TyagiVikas Tyagi Royal Oak, MI More articles by this author , Don BuiDon Bui Royal Oak, MI More articles by this author , Harvey QuHarvey Qu Rochester, MI More articles by this author , Kenneth PetersKenneth Peters Royal Oak, MI More articles by this author , Yao-Chi ChuangYao-Chi Chuang Kaohsiung, Taiwan More articles by this author , Hann-Chorng KuoHann-Chorng Kuo Hualien, Taiwan More articles by this author , Naoki YoshimuraNaoki Yoshimura Pittsburgh, PA More articles by this author , and Michael ChancellorMichael Chancellor Royal Oak, MI More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1368AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The symptoms of overactive bladder (OAB) overlap with symptoms of Interstitial Cystitis/Painful Bladder Syndrome (IC/PBS) and there is an absence of diagnostic tools for objective classification. Recent studies suggest association of inflammation with both diseases, and we hypothesized mechnastic differences in paracrine signaling mechanisms underlying OAB and IC/PBS are orchestrated by distinct chemokines or cytokines. METHODS Mid stream urine samples were obtained from well-characterized 39 IC/PBS patients and 17 OAB patients after informed consent. The IC/PBS patients in our study cohort were predominantly females and their age was stochastically lower than that of OAB patients. Samples were analysed for 20 cytokines, chemokines and growth factors using Luminex xMAP assay platform. The data on urinary proteins was analysed by univariate and multivariable analyses such as principal component analysis (PCA) to obtain distinct chemokine signatures of two diseases. RESULTS Out of the 20 inflammation associated proteins tested in urine only 15 were consistently detected. The PCA analysis identified principal drivers of variance and 90% of the variance in OAB group was explained by age, which is in agreement with higher prevalence of OAB in elderly population. No single protein among the 15 proteins consistently detected in urine could alone discriminate the two disease groups. Using forward selection and Akaike information criterion for variable selection and model comparison by logistic regression, the addition of 3 inflammatory proteins, namely MCP-1, IL-5 and eotaxin in the preferred model improved the prediction of IC/PBS over OAB. The model further predicts that rise in IL-5 and MCP-1 increased the odds ratio for IC/PBS, wheras rise in eotaxin increased the odds ratio for OAB by 16%(p=0.04). Urine levels of IL-5, and IL-8 were stochastically higher in IC/PBS patients (p < 0.0032) and levels of IP-10, IL-10 and eotaxin were higher in OAB patients (p< 0.0447). CONCLUSIONS The differential in the urinary chemokine repertoire associated with OAB and IC/PBS support our hypothesis of mechanistically distinct inflammatory pathways underlying the two diseases. We believe analysis of urine in larger cohort of OAB and IC/PBS patients followed longitudinally will reduce the number of variables required in our predictive model and further optimize this model. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e614-e615 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Pradeep Tyagi Royal Oak, MI More articles by this author Vikas Tyagi Royal Oak, MI More articles by this author Don Bui Royal Oak, MI More articles by this author Harvey Qu Rochester, MI More articles by this author Kenneth Peters Royal Oak, MI More articles by this author Yao-Chi Chuang Kaohsiung, Taiwan More articles by this author Hann-Chorng Kuo Hualien, Taiwan More articles by this author Naoki Yoshimura Pittsburgh, PA More articles by this author Michael Chancellor Royal Oak, MI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...