15792 β3-Tubulin knockdown impairs microtubule dynamics, induces cell cycle arrest, and decreases the spontaneous release of microvesicles in human skin malignant melanoma cells (A375)

Abstract

Background: Microvesicles (MVs), ranging in size from 100 nm to 1000 nm, play an important role in carcinogenesis by promoting angiogenesis and tumor metastasis, interfering with anti-tumor immunity, and inducing multidrug resistance. The release of MVs requires structural changes in microfilaments, intermediate filaments, and microtubules. Class III β-tubulin (β3-tubulin), one of the 7 β-tubulin isotypes, is a microtubule component involved in malignant transformation and cancer development. Herein, we characterize the effects of β3-tubulin knockdown on microtubule dynamics, cell cycle regulation, and MV formation in human melanoma cells.