1573 EXPRESSION PROFILING OF INFLAMMATION RELATED GENES IN BENIGN PROSTATIC HYPERPLASIA

Abstract

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research1 Apr 20111573 EXPRESSION PROFILING OF INFLAMMATION RELATED GENES IN BENIGN PROSTATIC HYPERPLASIA Grégoire Robert, Frank Smit, Jansen Kees, Tilly Aalders, Alexandre de la Taille, and Jack Schalken Grégoire RobertGrégoire Robert Bordeaux, France More articles by this author , Frank SmitFrank Smit Nijmegen, Netherlands More articles by this author , Jansen KeesJansen Kees Nijmegen, Netherlands More articles by this author , Tilly AaldersTilly Aalders Nijmegen, Netherlands More articles by this author , Alexandre de la TailleAlexandre de la Taille Créteil, France More articles by this author , and Jack SchalkenJack Schalken Nijmegen, Netherlands More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1609AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chronic prostatic inflammation could be a central mechanism in benign prostatic hyperplasia (BPH) progression and complication. Until today, taking prostatic biopsies remains the only way to diagnose prostatic inflammation, but it cannot be proposed to all BPH patients. Our objective was to study the mRNA expression level of several genes involved in the inflammatory and immune responses, to identify potential biomarkers and to investigate their protein expression in urine samples. METHODS Ninety BPH tissue samples were investigated in two steps. First a hypothesis was generated using a profiling procedure with a panel of 96 genes on an initial set of 30 BPH samples. Then the candidate biomarkers were validated on a large number of samples (n=90). A histological inflammation score based on the density and the confluence of the lymphoid nodules was taken as reference for the diagnosis of prostatic inflammation. The relative expression of the genes were calculated and compared with the histological score. Finally, the protein expression was assessed on 45 urine samples collected after digital rectal examination of the prostate. The protein expression was measured by ELISA and compared with the available clinical data. RESULTS Of the 96 investigated genes, 9 were significantly up-regulated in the high inflammation group of samples. Four of them were significantly correlated with the inflammation score: CCR7, CD40LG, CTLA4 and ICOS (the IL17 up-regulation was not significant). In the urine samples, ICOS protein expression was easily quantified by ELISA and was associated with a higher post-void residual (93.2 vs 37.2ml; p=0.020) and a lower maximum urinary flow rate (9.1 vs 12.7ml/s; p=0.033). CONCLUSIONS Four genes were significantly up regulated at the mRNA level in the prostatic tissue of patients with severe inflammation: CCR7, CD40LG, CTLA4 and ICOS. In urine, and at the protein level, ICOS was easily measured by ELISA and was associated with a higher post-void residual and a lower maximum urinary flow rate. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e631-e632 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Grégoire Robert Bordeaux, France More articles by this author Frank Smit Nijmegen, Netherlands More articles by this author Jansen Kees Nijmegen, Netherlands More articles by this author Tilly Aalders Nijmegen, Netherlands More articles by this author Alexandre de la Taille Créteil, France More articles by this author Jack Schalken Nijmegen, Netherlands More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...