156-P

Abstract

Aim Anorexia nervosa (AN) is a complex disorder with many genetic factors implicated in disease onset and pathology. In some cases the presence of a particular allele appears to modulate the immune response to certain exogenous antigens and protects the host from illness, whereas in other cases the host may mount an immune response against self-antigens. Our aim is to study the distribution of DPB1 ∗ alelles in the in Anorexia Nervosa. Methods We have been studied 18 restrictive AN, ANR: 10 compulsive AN, ANC, and a control group (n = 128) of the Castilla y Leon (Spain) population. DNA was extracted by resin balls with metallic core (kit DNA Direct II from Dynal), obtaining 1-4 mcg of DNA per sample. We have employed for our study SSP Dynal kits for low resolution for the locus DPB ∗ , with minor experimental changes. We have reduced the amount of DNA per tube to 2-4 ng and the concentration of the Taq Polymerase to 0,1 U/tube, instead of 100 ng of DNA and 0,4 U/tube of Taq Polymerase recommended by the kit instructions. The conditions of the PCR have been: 1) 94 °C, 2 min, 1 cycle. 2) 94 °C 10 s, 65 °C 60 s 10 cycles. 3) 94 °C 10 s, 61 °C 50 s, 72 °C 30 s 30 cycles. 4) 72 °C 3 min. The PCR products were revealed by agarose electrophoresis. Results The allele frequencies in our study in the control group were: HLA DPB1 ∗ 0101, 3,6%; DPB1 ∗ 0201, 13,6%; DPB1 ∗ 0202, 3,2%; DPB1 ∗ 0301, 10,6%; DPB1 ∗ 0401, 34,4 %; DPB1 ∗ 0402, 7,6%; DPB1 ∗ 0501, 1,8%; DPB1 ∗ 0601, 2,4%; DPB1 ∗ 0901, 2,2%; DPB1 ∗ 1001, 2,4%; DPB1 ∗ 1101, 7,6%; DPB1 ∗ 1301, 0,6%; DPB1 ∗ 1401, 1,2%; DPB1 ∗ 1501, 1,2%; DPB1 ∗ 1701, 2,8%. Conclusions No significative differences with our control and AN groups for these alleles were found.