1557 DEVELOPMENT OF A NOVEL METHOD FOR MEASURING SERUM OXALATE BY USING A TRIS(2,2`-BIPYRIDYL) RUTHENIUM(II) CHEMILUMINESCENCE ANALYSIS SYSTEM, AND ITS IMPORTANCE FOR UROLITHIASIS PATIENTS

Abstract

You have accessJournal of UrologyStone Disease: New Technology1 Apr 20131557 DEVELOPMENT OF A NOVEL METHOD FOR MEASURING SERUM OXALATE BY USING A TRIS(2,2`-BIPYRIDYL) RUTHENIUM(II) CHEMILUMINESCENCE ANALYSIS SYSTEM, AND ITS IMPORTANCE FOR UROLITHIASIS PATIENTS Yasuhiko Ito, Takahiro Yasui, Atsushi Okada, Zuo Li, Takahiro Kobayashi, Masahito Hirose, Shuzo Hamamoto, Yasunori Itoh, Shoichi Sasaki, and Kenjiro Kohri Yasuhiko ItoYasuhiko Ito Nagoya, Japan More articles by this author , Takahiro YasuiTakahiro Yasui Nagoya, Japan More articles by this author , Atsushi OkadaAtsushi Okada Nagoya, Japan More articles by this author , Zuo LiZuo Li Nagoya, Japan More articles by this author , Takahiro KobayashiTakahiro Kobayashi Nagoya, Japan More articles by this author , Masahito HiroseMasahito Hirose Nagoya, Japan More articles by this author , Shuzo HamamotoShuzo Hamamoto Nagoya, Japan More articles by this author , Yasunori ItohYasunori Itoh Nagoya, Japan More articles by this author , Shoichi SasakiShoichi Sasaki Nagoya, Japan More articles by this author , and Kenjiro KohriKenjiro Kohri Nagoya, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.3067AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Urinary and serum oxalate measurements are important for evaluating the pathogenesis and diagnosis of urolithiasis. Measuring serum oxalate is difficult because its density is much lower than that of urinary oxalate; many procedures have been reported for urinary oxalate measurement. We report the development of a method for measuring serum oxalate by using a tris(2,2`-bipyridyl) ruthenium(II) chemiluminescence analysis system. METHODS Measurement method: Blood specimens were centrifuged (2,000 × g, for 10 min) after standing at room temperature to separate serum. An equal amount of deproteinization liquid was added to each sample; the mixture was centrifuged (5,000 × g, 15 min) and the supernatant was collected. The samples were mixed with the tris(2,2`-bipyridyl) ruthenium(II) complex and light emission was measured using a light detector (FOM-110A, Hokuto Denko, Yokohama, Japan). The detectable oxalate range was 0.2-10 μmol/L. A fixed-quantity straight line was plotted by measuring the control oxalate solution and the samples were diluted 2, 4, and 8 times. This line was used to calculate the oxalate density of each specimen. Specimens: Blood samples were obtained from 50 patients with calcium oxalate urolithiasis (38 men, 12 women), 30 healthy men, and 2 patients with primary hyperoxaluria. RESULTS Sample preparation and measurement were rapid (20 minutes). The serum oxalate density was approximately constant at each dilution. The serum oxalate densities were 14.8 ± 6.1 μmol/L and 13.5 ± 5.7 μmol/L, respectively, for men and women with urolithiasis. The level in men with urolithiasis was significantly higher than that in men without urolithiasis (5.1 ± 1.5 μmol/L, p < 0.05). The serum oxalate density for the primary hyperoxaluria patients was 80-110 μmol/L, but fell to approximately 40 μmol/L at 2 months after liver and renal transplantation. CONCLUSIONS A novel serum oxalate measurement method was developed. The analytical characteristics of this chemiluminescence method indicated that it has high sensitivity, a wide range, few contamination problems, and a low background level. Serum oxalate levels were higher in urolithiasis patients than in healthy men. Serum oxalate measurement is useful not only as an index for primary hyperoxaluria treatment but also for the screening of and evaluating the disease pathogenesis of calcium oxalate urolithiasis. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e639 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yasuhiko Ito Nagoya, Japan More articles by this author Takahiro Yasui Nagoya, Japan More articles by this author Atsushi Okada Nagoya, Japan More articles by this author Zuo Li Nagoya, Japan More articles by this author Takahiro Kobayashi Nagoya, Japan More articles by this author Masahito Hirose Nagoya, Japan More articles by this author Shuzo Hamamoto Nagoya, Japan More articles by this author Yasunori Itoh Nagoya, Japan More articles by this author Shoichi Sasaki Nagoya, Japan More articles by this author Kenjiro Kohri Nagoya, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...