Abstract
152 Gadolinium-labeled liposomes as a DENSE-compatible, positive MRI contrast agent for serially assessing macrophage activity and regional contractile function in the murine heart after myocardial infarction
Highlights
Monocyte/macrophage activity in the left ventricle (LV) post-myocardial infarction (MI) has previously been assessed using small/ultra-small paramagnetic iron oxide (SPIO/USPIO) particles to yield negative contrast on CMR images
To develop a new multimodal, multilamellar liposomal agent labeled with both Gd-DTPA and a fluorescent tag (DiI-DS) that provides for serial macrophage tracking without interfering with cine DENSE analysis of contractile function
Quantitative analysis of total DiI fluorescence revealed that >95% of the MLLs were cleared from the blood by 4–6 h post injection. This notwithstanding, FACS indicated that the percentage of DiI-positive leukocytes in the blood remained fairly constant over this same period of
Summary
Monocyte/macrophage activity in the left ventricle (LV) post-myocardial infarction (MI) has previously been assessed using small/ultra-small paramagnetic iron oxide (SPIO/USPIO) particles to yield negative contrast on CMR images. The susceptibility-induced signal loss from iron interferes with the quantitation of myocardial strain in the very regions of interest occupied by the iron particles. To develop a new multimodal, multilamellar liposomal agent labeled with both Gd-DTPA (for CMR imaging) and a fluorescent tag (DiI-DS) that provides for serial macrophage tracking without interfering with cine DENSE analysis of contractile function
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