152. Effect of adrenalectomy on the secretion of IgA in the small intestine under acute stress

Abstract

It is known that stress causes significant changes on the immune system through the production of glucocorticoids and catecholamines. The objective of this study was to determined the effect of adrenalectomy on production and secretion of IgA pIgR in the small intestine of BALB/c under acute stress by immobilization 1 h. We considered 5 study groups: (1) control, (2) SHAM (surgery without adrenalectomy) (3) SHAM + E (with stress), (4) Adrenalectomized (ADX), and (5) Stressed Adrenalectomized (ADX + E). In all groups were determined the serum corticosterone levels by EIA, SIgA in intestinal fluid by ELISA and pIgR, IgA and J chain in epithelial cells by Western Blot. The results showed in group SHAM + E an increase in serum corticosterone levels, SIgA in intestinal fluid, IgA, J-chain and pIgR in epithelial cells; The corticosterone ADX levels were near zero; pIgR showed no difference from the control and can be directly related their production to hormonal stimulation. The changes in the morphology and physiology of intestinal epithelium in adrenalectomized mice without stress, favored the increase in the transport of IgA and J chain in epithelial cells, in stress conditions adrenalectomy undergoes further changes in the intestine, explaining why ADX + E group shows no change in the expression of IgA in epithelial cells compared to control groups. This work was supported by SIP-COFAA-IPN.