152: Development of DNA damage foci, loss of Lamin B and activation of pp38MAPK in pPROM: classic signs of senescence in human amniochorion

Abstract

Telomeres, guanine rich protective structures of chromosomes, are vulnerable to oxidative stress (OS) resulting in telomere dysfunction. These telomeres induce cell cycle arrest and senescence, characterized by DNA damage foci (DDF- phosphorylation of histone proteins [g-H2AX]) and loss of nuclear structural protein Lamin B. Telomere attrition and senescence are recently documented in preterm premature rupture of membranes (pPROM). Our objective was to examine the presence of oxidative stress, DDF and prosenescence markers in fetal membranes from pPROM.