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Abstract
Publisher Summary This chapter discusses purification of phospholipase C-activating G Protein G 11, from Turkey Erythrocytes. Turkey erythrocytes are an easily obtained homogeneous cell type which apparently express G 11 but not the similar (88% homologous) G q . These cells thus provides starting source for purification of a member of the Gq family. The turkey erythrocyte offers the further advantage that other protein components of the phosphoinositide-specific signal transduction pathway, including a G-protein-regulated PLC and G-protein βγ subunits, can be purified by relatively straightforward procedures. The purification of avian G 11 described in the chapter does not utilize βγ -affinity chromatography, a highly effective but specialized method of G-protein α-subunit purification that is not available in all laboratories. Rather, the procedure relies on conventional chromatographic techniques and monitors the capacity of column fractions to reconstitute AlF 4 - to purified turkey erythrocyte PLC.
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