147P - Serum netrin-1 as a biomarker for colorectal cancer detection

Abstract

BackgroundRecent evidence supported that netrin-1 involves in colorectal carcinogenesis. This study was to evaluate the performance of serum netrin-1 for detection of colorectal cancer (CRC) in both a clinical set and a screening set. MethodsA total of 115 consecutive patients with CRC and matched healthy controls were included in Clinical Set. Fifty subjects with CRC, 50 subjects with advanced adenoma (AA), and 150 matched control participants free of neoplasia were included in Screening Set. Circulating levels of netrin-1 were evaluated with commercial ELISA kits. ResultsIn Clinical set, subjects with CRC presented higher levels of serum netrin-1 (513.9±22.6 pg/mL) than controls (347.8±20.3 pg/mL, p<0.0001). Similar in Screening set, serum levels of netrin-1 was higher in CRC (644.5±37.0 pg/mL), in comparison with controls (407.7±14.8 pg/mL, p<0.0001) and AA (416.5±18.5 pg/mL, p<0.0001). However, there was no difference between controls and AA (p=0.752). Compared with the low netrin-1 group, the high group presented increased risk of CRC (Clinical set: OR=4.300 [95% CI 2.473 – 7.477], p<0.001); Screening set: OR=7.731 [95% CI 3.618 – 16.519], p<0.001). ROC curve of netrin-1 was developed to detect CRC (Clinical set: AUC 0.703 [95% CI 0.636 – 0.770]; Screening set: AUC 0.759 [95% CI 0.680 – 0.837]).Table: 147PStudy population characteristicsTable: 147PClinical setScreening setControlsCRCControlsAACRCNumber1151151505050Netrin-1 levels (pg/ml)347.8 ± 20.3*513.9 ± 22.6*407.7 ± 14.8#416.5 ± 18.5^644.5 ± 37.0#^Age (years)53.8 ± 8.755.9 ± 9.154.2 ± 9.251.9 ± 10.055.8 ± 9.6SexMale92921113737Female2323391313BMI (kg/m2)22.8 ± 3.024.6 ± 3.922.4 ± 2.923.1 ± 3.123.7 ± 3.1WHR0.89 ± 0.080.92 ± 0.060.88 ± 0.080.94 ± 0.090.99 ± 0.07TNM stageI1610II4322III4213IV145LocationColon8334Rectum3216Data are mean ± SD for continuous variables.*p<0.001; #p<0.001; ∧p<0.001. AA, advanced adenomas; BMI, body mass index; WHR, waist-to-hip circumference ratio. ConclusionsIt suggests netrin-1 as a potential biomarker in the screening and detection of CRC. Legal entity responsible for the studyThe First Affiliated Hospital of Soochow University. FundingJiangsu Provincial Key Research and Development Plan (No. BE2018659) and Provincial Key Laboratory Program of Higher Education (No. KJS1867). DisclosureAll authors have declared no conflicts of interest.