1472 EXPRESSION OF EPITHELIAL-MESENCHYMAL TRANSITION MARKERS IN LOCALIZED PROSTATE CANCER: IMPACT ON CLINICOPATHOLOGICAL OUTCOMES IN PATIENTS UNDERGOING RADICAL PROSTATECTOMY

Abstract

You have accessJournal of UrologyProstate Cancer: Localized VII1 Apr 20121472 EXPRESSION OF EPITHELIAL-MESENCHYMAL TRANSITION MARKERS IN LOCALIZED PROSTATE CANCER: IMPACT ON CLINICOPATHOLOGICAL OUTCOMES IN PATIENTS UNDERGOING RADICAL PROSTATECTOMY Hideaki Miyake, Hosny Behnsawy, and Masato Fujisawa Hideaki MiyakeHideaki Miyake Kobe, Japan More articles by this author , Hosny BehnsawyHosny Behnsawy Kobe, Japan More articles by this author , and Masato FujisawaMasato Fujisawa Kobe, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1993AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Radical prostatectomy (RP) is an effective therapeutic modality for men with clinically organ-confined prostate cancer; however, approximately 30% of patients will develop a postoperative biochemical recurrence (BR). The objective of this study was to evaluate the expression patterns of multiple molecular markers involved in the process of epithelial-mesenchymal transition (EMT) in prostate cancer to clarify the significance of these markers as predictors for BR in patients undergoing RP. METHODS This study included a total of 197 consecutive patients with clinically organ-confined prostate cancer who underwent RP between 2002 and 2008 without any neoadjuvant therapies. Expression levels of 13 EMT markers, including E-cadherin, N-cadherin, β-catenin, γ-catenin, fibronectin, matrix metalloproteinase (MMP)-2, MMP-9, Snail, Slug, Twist, vimentin, ZEB1 and ZEB2, in RP specimens obtained from these 197 patients were measured by immunohistochemical staining. These findings were analyzed according to several clinicopathological parameters. RESULTS Of the 13 markers, expression levels of E-cadherin, Snail, Twist and vimentin were significantly associated with several conventional prognostic factors. Univariate analysis identified these four EMT markers as significant predictors for BR as well, while serum prostate-specific antigen (PSA), Gleason score, seminal vesicle invasion (SVI), surgical margin status (SMS), lymph node metastasis and tumor volume were also significant. Of these significant factors, expression levels of Twist and vimentin, SVI, and SMS appeared to be independently related to BR by multivariate analysis. Furthermore, there were significant differences in BR-free survival according to positive numbers of these four independent factors; that is, BR occurred in 5 of 90 patients who were negative for risk factor (5.6%), 22 of 83 positive for a single or two risk factor (26.5%), and 19 of 24 positive for three or four risk factors (79.2%). CONCLUSIONS These findings suggest that consideration of expression levels of potential EMT markers in RP specimens, in addition to conventional prognostic parameters, would contribute to accurate prediction of BR following RP for localized prostate cancer. Moreover, combined evaluation of Twist and vimentin expression, SVI, and SMS would be particularly useful for further refinement of the system in predicting biochemical outcome following RP. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e597 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hideaki Miyake Kobe, Japan More articles by this author Hosny Behnsawy Kobe, Japan More articles by this author Masato Fujisawa Kobe, Japan More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...