147 EFFECTS OF FATTY ACIDS ON DEVELOPMENT OF RENAL CELL CARCINOMA BY UP-REGULATING INTEGRIN-LINKED KINASE IN VITRO

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research I1 Apr 2012147 EFFECTS OF FATTY ACIDS ON DEVELOPMENT OF RENAL CELL CARCINOMA BY UP-REGULATING INTEGRIN-LINKED KINASE IN VITRO Tao Xu, Zhenhua Liu, and Xiaofeng Wang Tao XuTao Xu Beijing, China, People's Republic of More articles by this author , Zhenhua LiuZhenhua Liu Beijing, China, People's Republic of More articles by this author , and Xiaofeng WangXiaofeng Wang Beijing, China, People's Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.197AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES An increased risk of renal cell carcinoma (RCC) has been linked with obesity and metabolic syndrome. But the mechanisms of lipid metabolic disorder affect the development of RCC is still not completely understood and highly controversial. We investigated whether dietary fatty acids promote RCC progression by up-regulating integrin-linked kinase (ILK). METHODS Effects of different fatty acids were assessed using 786-O cell (Renal clear cell carcinoma) and GRC-1 cell (Renal granular cell carcinoma). MTT colorimetric assay and the Transwell assay were performed respectively for cell proliferation and migration after treatment with different doses of oleic acid, palmitic acid and linoleic acid for 48h. The expression of ILK and NFκB were detected by Western blot. RESULTS The oleic acid and palmitic acid promoted proliferation of the two kinds cells in a dose-dependent manner, whereas the linoleic acid didn't showed similar effects in both of cell lines, compared with blank control. The effects of three fatty acids on cells migration were not significant. After treatment with oleic acid and palmitic acid, the expression of ILK and NFκB were increased in both 786-O cell and GRC-1 cell. CONCLUSIONS These data showed that certain fatty acids could promote the development of renal cell carcinoma via up-regulating ILK/NFκB pathway, which may be reveals the relations between metabolic disturbance and renal carcinoma to a certain extent. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e61 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tao Xu Beijing, China, People's Republic of More articles by this author Zhenhua Liu Beijing, China, People's Republic of More articles by this author Xiaofeng Wang Beijing, China, People's Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...