1443 THE IMPORTANT ROLE OF GLYCINE N-METHYLTRANSFERASE (GNMT) IN THE CARCINOGENESIS AND PROGRESSION OF PROSTATE CANCER

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research1 Apr 20111443 THE IMPORTANT ROLE OF GLYCINE N-METHYLTRANSFERASE (GNMT) IN THE CARCINOGENESIS AND PROGRESSION OF PROSTATE CANCER YooHyun Song, Masaki Shiota, Kentaro Kuroiwa, Seiji Naito, and Yoshinao Oda YooHyun SongYooHyun Song Fukuoka, Japan More articles by this author , Masaki ShiotaMasaki Shiota Vancouver, Canada More articles by this author , Kentaro KuroiwaKentaro Kuroiwa Fukuoka, Japan More articles by this author , Seiji NaitoSeiji Naito Fukuoka, Japan More articles by this author , and Yoshinao OdaYoshinao Oda Fukuoka, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1356AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES GNMT plays a role in the metabolism of methionine as well as in gluconeogenesis. It has recently been reported that the GNMT gene acts as a tumor-susceptible gene. However, little is known about the specific function of GNMT in carcinogenesis and malignant progression. This study aimed to investigate the function of GNMT in prostate cancer. METHODS We used siRNAs to examine the effects of GNMT knockdown on cell proliferation and the cell cycle. In addition, the relation between immunohistochemical GNMT expression and clinicopathologic parameters was investigated in 148 prostate cancer tissues. RESULTS We show that siRNA-mediated GNMT knockdown results in an inhibition of proliferation, and induces G1 arrest and apoptosis in prostate cancer cell lines. Moreover, high cytoplasmic GNMT expression was also correlated with a higher Gleason score (p = 0.001) and higher pT stage (p = 0.0459). The patients with high GNMT cytoplasmic expression showed significantly lower disease-free survival rates than patients with low expression (p = 0.0007). High GNMT cytoplasmic expression had a significant impact on patient disease-free survival in multivariate analysis (p = 0.0052). CONCLUSIONS This is the first investigation to reveal the novel finding that GNMT may play an important role in promoting prostate cancer cell growth via the regulation of apoptosis and contribute to the progression of prostate cancer. The modulation of GNMT expression or function may be a strategy for developing novel therapeutics for prostate cancer. GNMT may represent a novel marker of malignant progression and poor prognosis in prostate cancer. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e578-e579 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information YooHyun Song Fukuoka, Japan More articles by this author Masaki Shiota Vancouver, Canada More articles by this author Kentaro Kuroiwa Fukuoka, Japan More articles by this author Seiji Naito Fukuoka, Japan More articles by this author Yoshinao Oda Fukuoka, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...