Abstract
Publisher Summary This chapter describes the isolation procedure and properties of nekoflavin. Nekoflavin has the general properties of a flavin and is found in cat eyes. Flavins are extracted from the choroids of cat eyes with dilute perchloric acid and are adsorbed on florisil. They are eluted with dilute pyridine. It is reduced by sodium dithionite losing fluorescence and is reoxidized by oxygen restoring fluorescence. The absorption spectrum is very similar to that of riboflavin. Nekoflavin is separated from riboflavin and purified by column chromatography on powdered cellulose. The first solvent system is n -butanol-formic acid-water, the second is n- butanol-formic acidwater- ether, and the third is isoamyl alcohol-methyl ethyl ketone-acetic acid-water. A flavin compound has the same R f values in chromatography as nekoflavin. Thin-layer chromatography on silica gel is suitable for the identification of nekoflavin. Paper chromatography is also useful. The chromatography of nekoflavin and the oxidation product of nekoflavin with periodate is important, because the reaction product of nekoflavin is different from that of ordinary riboflavin derivatives.
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