Abstract
The purpose of this study is to assess the vitrified artificial tissue with the effective thermal diffusivity. Human fibroblast cultured into collagen sponge was used for the test artificial tissue, frozen at a cooling rate of 30∿1200℃/min from room temperature to liquid-nitrogen temperature (-196℃). The test chamber (Φ24×4mm) for freezing the test artificial tissue was in contact with a carbon heater that was on a copper block, which was immersed in liquid-nitrogen. The temperature of the test artificial tissue was increased stepwise from -190℃ to -185℃, and then the effective thermal diffusivity was deter-mined by Laplace transform method. After the measurement of the effective thermal diffusivety, post-thaw viability of human fibroblast was measured by trypan blue exclusion assay. As a result, the effective thermal diffusivity decresed and the viability increased with increasing the cooling rate. The decrease of the effective thermal diffusivity and the increase of the viability show the decrease of quantity of intra-and extracellular ice formation in the artificial tissue. Our results demonstrate that the vitrified artificial tissue can be assessed with the effective thermal diffusivity.
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