141: Glutamine treatment attenuates hyperglycemia induced mitochondrial stress and apoptosis in umbilical vein endothelial cells: A relevance in severe sepsis

Abstract

Purpose The aim of this study was to determine the invitro effect of glutamine and insulin on apoptosis, mitochondrial membrane potential, cell permeability and inflammatory cytokines in hyperglycemic umbilical vein endothelial cell. Materials and methods In our study, human umbilical vein endothelial cells were grown and subjected to hyperglycemia and the effect of glutamine and insulin. Mitochondrial functions and the production of inflammatory cytokines were determined using florescence analysis and multiple cytotoxicity assays. Apoptosis was analyzed by the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Results Our results demonstrate that glutamine maintains the integrity of the mitochondria by reducing the cell permeability and cytochrome c intensity, and increasing the mitochondrial membrane potential. Cytochrome c intensity was significantly ( p p p p Conclusion Our data conclude that glutamine alone or in combination with insulin can positively disrupt mitochondrial stress and cell permeability in umbilical vein endothelial cell under hyperglycemic conditions. At the same time our data support that glutamine regulates the expression of inflammatory cytokines and maintains the balance of the mitochondria in a cytoprotective manner, whose dysfunction in the hyperglycemic endothelial cells may reflect the degree of systemic injury in severe sepsis.