140. Inflammation and sickness induced by lipopolysaccharide in adult and aged mice are not mitigated by dietary broccoli

Abstract

Aging is associated with oxidative stress and heightened inflammatory response to infection. Dietary interventions to reduce these changes are therefore desirable. Broccoli contains glucoraphanin, which is converted to sulforaphane (SFN) during digestion. SFN increases antioxidants including NAD (P) H quinone oxidoreductase (NQO1) and inhibits inflammatory cytokines. We hypothesized that dietary broccoli would support an antioxidant response in brain and periphery and inhibit LPS-induced inflammation and sickness. Adult and aged mice were fed control or 10% broccoli diet for 4 wk prior to an LPS injection. Social interactions were assessed 2, 4, 8, and 24 h following LPS, and mRNA quantified in liver and brain at 24 h. Dietary broccoli did not prevent LPS-induced decrease in social interactions in young or aged mice. Interleukin (IL)-1β expression was unaffected by broccoli consumption and was induced by LPS in brain and liver of adult and aged mice (p