1,4-二氯苯的細胞毒性

Abstract

l,4-dichlorobenzene (DCB), used as an ingredient in indoor deodorants, might be hazardous to workers in synthesizing or packaging facilities. 1,4-DCB can be metabolized into 2,5-dichlorophenol (DCP) after entering the human body through the skin or orally via contaminated food and drink. The cytotoxicity of 1,4-DCB, compared with 2, 5-DCP, was examined in human cell lines, including lung adenocaricinoma H1355 cells, hepatoma HepG2 cells, HeLa cells and leukemia Jurkat cells. Trypan blue staining assay was used to detect the cytotoxicity of 30-minute treatment, and caspase/PI detections was used to distinguish apoptosis and necrosis. The LD(subscript 50S) of 1, 4-DCB were 1.02, 1.02, 1.42 and 1.18 mM in lung adenocaricinoma H1355 cells, hepatoma HepG2 cells, leukemia Jurkat cells and HeLa cells. Compared with 1, 4-DCB, 2, 5-DCP had higher LD50 as 2.03 mM in leukemia Jurkat cells. The extent of apoptosis and necrosis in human leukemia Jurkat cells was analuzed after the cells had been treated with 1, 4-DCI3 for 24 hours. A very high percentage of apoptosis was gained (around 29%) in the presence of 1, 4-DCB above 700µM. When apoptosis was analyzed by a mitosensor to measure the mitochondria membrane potential in HeLa cells, 500µM 1, 4-DCB caused 20% of the cells to lose their mitochondria membrane potential.