Abstract
Publisher Summary The collection of lipid monolayers provides a simple, but effective, method for the determination of reactant distribution or adsorbed lipase in monomolecular films. Monolayer collection has been applied in two variations. One is based on aspiration of the surface phase 9–12 and the second on the adsorption of the surface phase to a hydrophobic support. Although conceptually identical, there are practical differences in the data obtainable with each of these techniques. The first is sensitivity for determining net lipase adsorption. Sensitivity of the measurements is limited by the volume of the aqueous subphase that is adventitiously collected with the monolayer. When lipase adsorption is relatively low and subphase enzyme concentration is relatively high, the correction for enzyme carried over in the aqueous subphase can greatly exceed the quantity of adsorbed lipase, especially with the aspiration approach.
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