Abstract
The introduction of differential display (DD) and related techniques has contributed to the recent shift in focus from DNA genetics to expression genetics, especially in the field of cancer research. This chapter describes the principles of DD, including specific methods for working with the primers that have been used extensively. The chapter discusses the production of a DD gel, strategies for isolating and identifying DD band cDNAs, and information on performing high-throughput DD. DD is distinguished from related methods by a low stringency, competitive polymerase chain reaction (PCR) step that uses primer pairs to target the 3’ ends of messenger RNAs. One of the major criticisms of the DD technique has been the high number of false positives obtained in some studies.
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