Abstract
Publisher Summary This chapter describes the procedure for the induction of immune interferon from human mononuclear cells by the combined treatment with OK-432 and staphylococcal enterotoxin B (SEB) in serum-free medium. A high titer of immune interferon can be obtained, because OK-432 and SEB act synergistically on the induction of immune interferon. Antiviral assay of immune interferon is performed by a cytopathic effect inhibition assay with sindbis virus and FL amnion cells. Units are determined with respect to a laboratory reference preparation (crude immune interferon), which is titrated against the human leukocyte interferon reference standard from the National Institutes of Health. For induction of immune interferon, human mononuclear cells are cultured in the serum-free induction medium. Blood is collected in heparin by venipuncture from healthy donors. In this combination system, human immune interferon can be obtained with a relatively high titer, especially from low responder cells. Almost all the interferon activity is neutralized by anti-IFN-γ serum, whereas anti-IFN-α serum or anti-IFN-β serum has no effect on interferon activity. The purification procedure for immune interferon is simplified, because the induction medium contains human serum albumin instead of serum.
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