14] Induction and production of human interferon with a continuous line of modified fibroblasts

Abstract

Publisher Summary The production of human fibroblast type interferon depends on the innovative induction procedures to increase production rather than on large-scale cell culture techniques as in the case of lymphoblastoid cell cultures. Interferon induction in transformed or modified human fibroblasts is described. It is important to note that, unlike the primary diploid fibroblast cultures, the modified human fibroblast cultures are capable of producing human interferon in response to a large number of substances structurally unrelated to the traditional inducer-type molecule like poly(I).poly(C). Various conditions are used to induce C-10 cell cultures optimally for interferon production on a large (100-200 flasks) scale including approximately 3 x l0 6 C-10 cells being seeded into each 175-cm 2 plastic culture flask; before inducing the C-10 cells, each culture is primed with 250 units/ml of partially purified human fibroblast interferon (specific activity of 1 x 10 6 units/rag protein) for 10-12 hr and the culture fluid is harvested at the end of 16 hour.