Abstract
This chapter describes a method for continuous production of polyacrylamide-entrapped enzyme beads. The immobilization of enzymes by entrapping them into polyacrylamide gel has been proved efficient and convenient for many enzymes. In most cases, the enzyme gel is produced by block polymerization with subsequent disruption of the formed gel. The gel particles produced are irregular in form and size. These irregular particles cause high hydraulic resistance in packed beds and the gel in batch reactors wears out quickly. Exact kinetic measurements are also difficult under these conditions. The production of polyacrylamide gel in micro spherical form by dispersing the polymerizable solution into an organic solvent immiscible in water is well known. The same method has been employed for polymerizing enzyme-containing solutions. Trypsin entrapped in spherical beads of gel showed higher activity than that in irregular particles of block gel. To increase the productivity of bead polymerization and to regulate gel size, the chapter has employed a special apparatus for carrying out this process.
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