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Abstract
13C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells
Highlights
Isotope labeling techniques have become a most valuable tool in metabolomics and fluxomics [1]
Aspartate, and glutamine were only consumed, we found significant extracellular labeling of these metabolites indicating simultaneous consumption and excretion
Significant labeling of serine and glycine was found on labeled glucose but not on labeled glutamine indicating the absence of gluconeogenesis
Summary
Isotope labeling techniques have become a most valuable tool in metabolomics and fluxomics [1]. A thorough understanding of CHO metabolism is crucial for metabolic engineering and process optimization
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