139 Identification of long noncoding RNAs in mouse hair follicle stem cells using computational methods

Abstract

Gene expression profiling, especially deep sequencing, has contributed significantly to our understanding of hair follicle stem cell biology; however, roles played by non-coding RNAs, especially the long non-coding RNAs (lncRNAs) for the most part remain undefined. We performed Illumina paired end sequencing and received raw reads for stem- and non-stem cell samples. FASTQC quality control was performed and adaptors were trimmed using Trimmomatic. We aligned the reads to the reference genome using Tophat.