Abstract
Datawere analyzed by regression analysis and by a random effects mixedmodel. Therewere significant (P < 0.001) effects of time and assay method for the determination of progestins in the mixed model. Least squares means for progestins across the studied interval differed (P < 0.001) with P4 1⁄4 0.66 ng/mL; DHP 1⁄4 26.4 ng/ mL; ELISA progestins 1⁄4 47.9 ng/mL and mini-Vidas progestins 1⁄4 20.8 ng/mL (pooled SEM 1⁄4 0.66). These data confirm progestin determination by immunoassay during late gestation varies depending upon the assay used and the apparent cross reactivity to other progestins present in high concentrations during late gestation in the mare. More widespread application of LC-MS/MS for determination of progestins in the late pregnant mare will enhance the interpretation of progestin concentrations. Study was supported by the Albert G. Clay Endowment. Biom erieux provided the progestin immunoassays for the mini-Vidas.
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