1375 FUNCTIONAL ANALYSIS OF SPERMATOGONIAL STEM CELLS IN CRYPTORCHIDISM USING EEF1A1 AND TPT1 GENE EXPRESSION AS INDEXES OF CELL DIFFERENTIATION

Abstract

You have accessJournal of UrologyPediatrics: Andrology - Cryptorchidism & Varicoceles1 Apr 20121375 FUNCTIONAL ANALYSIS OF SPERMATOGONIAL STEM CELLS IN CRYPTORCHIDISM USING EEF1A1 AND TPT1 GENE EXPRESSION AS INDEXES OF CELL DIFFERENTIATION Kentaro Mizuno, Yoshiyuki Kojima, Hideyuki Kamisawa, Satoshi Kurokawa, Makoto Imura, Yoshinobu Moritoki, Hidenori Nishio, Yasuhiro Shibata, Akihiro Nakane, Toshiki Kato, Tetsuji Maruyama, Yutaro Hayashi, and Kenjiro Kohri Kentaro MizunoKentaro Mizuno Nagoya, Japan More articles by this author , Yoshiyuki KojimaYoshiyuki Kojima Nagoya, Japan More articles by this author , Hideyuki KamisawaHideyuki Kamisawa Nagoya, Japan More articles by this author , Satoshi KurokawaSatoshi Kurokawa Nagoya, Japan More articles by this author , Makoto ImuraMakoto Imura Nagoya, Japan More articles by this author , Yoshinobu MoritokiYoshinobu Moritoki Nagoya, Japan More articles by this author , Hidenori NishioHidenori Nishio Nagoya, Japan More articles by this author , Yasuhiro ShibataYasuhiro Shibata Nagoya, Japan More articles by this author , Akihiro NakaneAkihiro Nakane Nagoya, Japan More articles by this author , Toshiki KatoToshiki Kato Nagoya, Japan More articles by this author , Tetsuji MaruyamaTetsuji Maruyama Nagoya, Japan More articles by this author , Yutaro HayashiYutaro Hayashi Nagoya, Japan More articles by this author , and Kenjiro KohriKenjiro Kohri Nagoya, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1759AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It has been suggested that future fertility is affected by the number or function of spermatogonia derived from spermatogonial stem cells in cryptorchid testes. We have previously found that EEF1A1 and TPT1 genes are differentially expressed in human cryptorchid testes and believe that these genes reflect the stage of spermatogonia development. In this context, we have previously evaluated the expression of these genes in boys aged 1–5 years, but expression in other age groups remains unclear. To elucidate the chronological differentiation in spermatogonia, we assessed cryptorchid testes by histological and molecular biological examination. METHODS We used specimens derived from testicular biopsy during orchiopexy for cryptorchidism (88 cases, 109 testes) from October 2001 to April 2011. The patients were divided into 2 groups:(1) early orchiopexy (before 1 year of age) and (2) late orchiopexy (after 6 years of age). Histological assessment using light microscopy and molecular biological assessment using the expression levels of EEF1A1 and TPT1 genes by real-time RT-PCR were performed. Relative expression values of these genes were calculated and compared with those from age-matched descended testes. RESULTS The number of spermatogonia per tubule was significantly higher in the early orchiopexy group (p < 0.005). Relative expression values of the EEF1A1 gene were not significantly different between groups, whereas those of the TPT1 gene in the late orchiopexy group were significantly lower than those of age-matched descended testes (p < 0.005). CONCLUSIONS It has been suggested that leaving cryptorchid testes until school age induces testicular fibrillization and loss of spermatogonia. Combined with our previous results, it can be seen that the EEF1A1 gene is highly expressed until 6 years of age and is subsequently equally expressed in cryptorchid testes and descended testes. In contrast, expression of the TPT1 gene was lower in cryptorchid testes than descended testes after 6 years of age. The TPT1 gene was expressed in spermatogonia and was associated with an undifferentiated state of these cells, suggesting that spermatogonia could not maintain stemness in cryptorchid testes. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e558-e559 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kentaro Mizuno Nagoya, Japan More articles by this author Yoshiyuki Kojima Nagoya, Japan More articles by this author Hideyuki Kamisawa Nagoya, Japan More articles by this author Satoshi Kurokawa Nagoya, Japan More articles by this author Makoto Imura Nagoya, Japan More articles by this author Yoshinobu Moritoki Nagoya, Japan More articles by this author Hidenori Nishio Nagoya, Japan More articles by this author Yasuhiro Shibata Nagoya, Japan More articles by this author Akihiro Nakane Nagoya, Japan More articles by this author Toshiki Kato Nagoya, Japan More articles by this author Tetsuji Maruyama Nagoya, Japan More articles by this author Yutaro Hayashi Nagoya, Japan More articles by this author Kenjiro Kohri Nagoya, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...