135 Comparison of the Expression of CD11B Receptors of Neutrofils in Preterm Neonates and Term Healthy Neonates Assessed in Umbilical Artery Blood.

Abstract

Objectives: An intrauterine infection of bacterial origin is the most frequent cause of preterm births. Promising marker of the intrauterine infection in preterm neonates seems to be an assessment of the expression of CD11b receptors of neutrofils. Aim of the work: To compare the expression of CD11b receptors of neutrophils assessed in umbilical artery blood in preterm neonates and healthy term neonates. Material and methods: Expression of CD11b receptors of neotrophils was assessed in umbilical artery blood by flow cytometric analysis in 17 preterm neonates (average gestational age - 28,9 week) and 11 healthy term neonates (average gestational age - 38,9 wek). Additionally, in group of preterm neonates the expression of CD11b receptors of neutrophils was assessed twice, every 24 hours, in 2nd and 3rd day of life. Expression assessment was done with FACScalibur BD analyser and with CD11b (BD) monoclonal antibodies. For statistical significance p<0,01 level was admitted. Results: There was no statistically significant difference between the expression of CD11b receptors of neutrophils in umbilical artery blood in group of preterm neonates and term healthy neonates (p=0,3930). However, increase of the expression of CD11b receptors of neutrophils in group of preterm neonates between 1st day of life and 2nd and 3rd day of life (p<0,0000004 and p<0,00015 respectively) was statistically significant. Conclusions: 1. Expression of CD11b receptors of neutrophils assessed in umbilical artery blood in preterm neonates does not show a statistical significant difference in comparison to term healthy neonates. 2. Statistically significant increase of the expression of CD11b receptors of neutrophils in preterm neonates in the 2nd and 3rd day of life in comparison to the 1st day of life can be consider as a marker of the inflammation due to an intrauterine infection, but assessed only after 24 hours of life of preterm neonate.