1337 LONG NONCODING RNA-MEDIATED REGULATION OF BCL-2 EXPRESSION IN PROSTATE CANCERS

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research (VI)1 Apr 20131337 LONG NONCODING RNA-MEDIATED REGULATION OF BCL-2 EXPRESSION IN PROSTATE CANCERS Daniel Zainfeld, Ruitao Zhang, J Brantley Thrasher, and Benyi Li Daniel ZainfeldDaniel Zainfeld Kansas City, KS More articles by this author , Ruitao ZhangRuitao Zhang Kansas City, KS More articles by this author , J Brantley ThrasherJ Brantley Thrasher Kansas City, KS More articles by this author , and Benyi LiBenyi Li Kansas City, KS More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.2691AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The BCL-2 protein has been associated with the development of androgen independent prostate cancer as high levels of expression are present in castration-resistant tumors. Long non-coding RNAs (ncRNAs), though constituting a large portion of the mammalian transcriptome are not well understood in terms of their role in the development of prostate cancer. We attempted to elucidate an association between specific ncRNAs and BCL-2 expression. METHODS The expression patterns of mRNA and ncRNAs in normal prostatic tissue were compared to that of tumors of the prostate. Real-time quantitative (RT-PCR) analysis was used to quantify these expression patterns. Secondarily, RNAi technique was used to knock down ncRNA expression in prostate cancer cells in order to observe its effect on BCL-2 expression. RESULTS 4 unique ncRNAs were identified in association with the BCL-2 gene. In addition, these ncRNAs were overexpressed in prostatic tumors in comparison to matched non-malignant prostate compartments. Real-Time quantitative RT-PCR analysis indicated that BCL-2 mRNA and ncRNA-3 are highly over-expressed in parallel with a high degree of correlation in 9 prostate cancer cell lines (Correlation coefficient=0.732, P value=0.025), 35 prostate tumors (Correlation coefficient=0.954, P value=0.000) and non-malignant prostate tissues (Correlation coefficient=0.914, P value=0.000). Bcl-2 expression decreased dramatically following ncRNA-3 siRNA transfection. CONCLUSIONS ncRNA expression is altered in the setting of prostate cancer and may play an important role in the development of prostate cancer. Further mechanistic study is desirable and will further assist in understanding the role of ncRNAs in prostate cancer. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e546 Peer Review Report Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Daniel Zainfeld Kansas City, KS More articles by this author Ruitao Zhang Kansas City, KS More articles by this author J Brantley Thrasher Kansas City, KS More articles by this author Benyi Li Kansas City, KS More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...