1331-P: Associations between Insulin Resistance and Plasma Lipids and Metabolomics in Women with and without Type 1 Diabetes

Abstract

Insulin resistance (IR) increases risk for cardiovascular disease and mortality in people with type 1 diabetes (T1D) . We previously found associations between plasma lipids, metabolites and IR; whether these associations differ in people with and without T1D is not well understood. We analyzed targeted plasma lipidomics and untargeted plasma metabolomics in 25 premenopausal women aged 18-45 yrs (12 with T1D and 13 without diabetes [non-DM]) who had a hyperinsulinemic euglycemic clamp to measure glucose infusion rate (GIR) . After stratifying by diabetes, women were classified by tertiles of GIR. We used sparse partial least-squares discriminant analysis to predict and moderated t-tests to compare most IR vs. most insulin sensitive (IS) tertile. Among women with T1D, 3 lipids, 4 amino acids, 3 peptides, 3 xenobiotics, 1 carbohydrate, and 1 nucleotide were lower and 1 lipid and 1 fatty acid were higher in the most IS tertile (Table) . Among non-DM women, 4 lipids, 1 cofactor, 1 fatty acid, and 1 phosphatidylethanolamine were lower and 1 nucleotide and 1 lipid were higher in the most IS tertile. None of the top features were the same in the T1D and non-DM groups. Plasma lipids and metabolites were differentially associated with IR in women with and without T1D. Future work should validate these findings in a larger sample of women and men, and explore how these biomarkers relate to IR and metabolism in T1D. Disclosure T.B.Vigers: None. L.Pyle: None. I.E.Schauer: None. B.C.Bergman: Research Support; Eli Lilly and Company. J.K.Snell-bergeon: Stock/Shareholder; GlaxoSmithKline plc. Funding American Diabetes Association (7-13-CD-10) ; National Heart, Lung, and Blood Institute (grant HL61753 and HL113029) , JDRF Diabetes Foundation (grant 17-2013-313) , DERC Clinical Investigation Core (grant P30 DK57516) , NIH-M01-RR00051, NIH P30-DK116073