Abstract
Introduction: We previously reported to this forum and published, down-regulation of the growth-suppressor gene, cyclin-dependent kinase 2- associated protein 1 (CDK2-AP1) in human (MSI) colorectal cancer cell lines is associated with increased proliferation and decreased apoptosis in those cell lines. In this report we expand our study of CDK2-AP1 to include a mouse model of MSI CRC and a human CRC tissue array to validate our original cell line based observations. Methods: The DNA mismatch repair (MMR) status of tumors arising from a knockout mouse, generated in our collaborator’s laboratory (Mlh1−/−/APC1638N), was determined by measuring Mlh1 expression by Western Blot and confirmed by sequencing four mouse microsatellite markers (D9Mit67, D1Mit79, L24372 and U12235).
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