1312 STIMULATION OF MACROPHAGE INHIBITORY CYTOKINE-1 BY PALMITIC ACID ACTIVATES PROSTATE CANCER CELL INVASIVENESS

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research II1 Apr 20121312 STIMULATION OF MACROPHAGE INHIBITORY CYTOKINE-1 BY PALMITIC ACID ACTIVATES PROSTATE CANCER CELL INVASIVENESS Huang Mingguo, Shintaro Narita, Shinya Maita, Kazuyuki Numakura, Takashi Obara, Takamitsu Inoue, Yohei Horikawa, Shigeru Satoh, and Tomonori Habuchi Huang MingguoHuang Mingguo Akita, Japan More articles by this author , Shintaro NaritaShintaro Narita Akita, Japan More articles by this author , Shinya MaitaShinya Maita Akita, Japan More articles by this author , Kazuyuki NumakuraKazuyuki Numakura Akita, Japan More articles by this author , Takashi ObaraTakashi Obara Akita, Japan More articles by this author , Takamitsu InoueTakamitsu Inoue Akita, Japan More articles by this author , Yohei HorikawaYohei Horikawa Akita, Japan More articles by this author , Shigeru SatohShigeru Satoh Akita, Japan More articles by this author , and Tomonori HabuchiTomonori Habuchi Akita, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1659AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Eating habits play important roles in prostate cancer (PCa) progression. Although lipid metabolism is strongly associated with diet-induced PCa progression, the type of fatty acid that has the most significant influence on PCa progression remains unknown. We investigate the role of long-chain fatty acids and their associated signals on PCa cell progression in vitro and in vivo. METHODS The impact of long-chain fatty acids, including palmitic acid (PA), oleic acid (OA), and linoleic acid (LA), on the proliferation and invasion of three PCa cells was assessed by cell proliferation and Matrigel invasion assays. Following stimulation by PA, the secretion and expression of transforming growth factor-beta family genes and proteins in PCa cells, other urological cancer cells, and benign prostate cells were measured using qRT-PCR, western blotting, and ELISA. The association between tumor volume and macrophage inhibitory cytokine-1 (MIC-1) expression in mouse xenograft fed with high-fat diet (HFD) or low-fat diet (LFD) was investigated. RESULTS There were no significant differences in cell proliferation in the three PCa cells treated with PA, OA, LA, and control. The three long-chain fatty acids significantly increased the invasive capacity of PC-3 and DU145 cells compared with control and the mean numbers of invaded cells were highest in PC-3 and DU145 cells treated with PA (p < 0.01 and p < 0.01, respectively). Among various cytokines, PA stimulated MIC-1 expression in the culture medium and cell extracts of three PCa cells, whereas there was no significant difference in benign prostate cells and other urological cancer cells. Furthermore, the invasive capacity of PC-3 and DU145 cells were significantly increased by treatment with recombinant MIC-1. The mean tumor volume in vivo in the HFD group was significantly higher than that in the LFD group (2569.9 vs 2010.3 mm3, p = 0.025). The mean level of serum MIC-1 in the HFD group was significantly higher than that in the LFD group (24155.2 vs 18343.8 pg/mL, p = 0.028). CONCLUSIONS Long-chain fatty acids, particularly PA, influence PCa cell invasiveness. Stimulation of MIC-1 by PA may be a significant underlying mechanism of diet-induced PCa progression. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e532 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Huang Mingguo Akita, Japan More articles by this author Shintaro Narita Akita, Japan More articles by this author Shinya Maita Akita, Japan More articles by this author Kazuyuki Numakura Akita, Japan More articles by this author Takashi Obara Akita, Japan More articles by this author Takamitsu Inoue Akita, Japan More articles by this author Yohei Horikawa Akita, Japan More articles by this author Shigeru Satoh Akita, Japan More articles by this author Tomonori Habuchi Akita, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...