Abstract
In this study, it is hypothesized that initial geometrical shapes of cutaneous wound and the major axis of the wound to the direction of stretch could influence myofibroblast realignment observed in the maturation phase of healing process. Firstly, human dermal fibroblasts were differentiated into myofibroblasts with a treatment of TGF-β1. The cells were then seeded onto a PDMS chamber either in a monolayer or in a colony of the following shapes: circle, and track fields both parallel and vertical to the stretch. For mechanical tests, cyclic tensile stretch, with an amplitude of 20%, was provided at 1 Hz for 6 hours. Myofibroblasts seeded in the circle and the parallel track exhibited a similar response of realigning ±70℃ to the strain axis with uniformly distributed myofibroblasts in the whole area. By contrast, the cells seeded in the shape of vertical track demonstrated realignment to no specific direction. Findings supports our hypothesis, indicating that the shape and the orientation of wound influences healing processes through myofibroblasts realignment.
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